Sensitivity of the response of cytosolic calcium in Quin-2-loaded rat hepatocytes to glucagon, adenine nucleosides, and adenine nucleotides.

@article{Sistare1985SensitivityOT,
  title={Sensitivity of the response of cytosolic calcium in Quin-2-loaded rat hepatocytes to glucagon, adenine nucleosides, and adenine nucleotides.},
  author={F D Sistare and Rich Picking and Robert C. Haynes},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 23},
  pages={12744-7}
}
Hepatocytes from fasted rats were used to study the effect of glucagon on intracellular free cytosolic Ca2+ ([Ca2+]i) indicated by the use of Quin-2-calcium fluorescence. It was found that, in both male and female rats, glucagon increased [Ca2+]i at a half-maximally effective concentration (Kact) of 0.3 nM, a concentration known to be half-maximal for… CONTINUE READING