Sensitivity of acid-fast staining for Mycobacterium tuberculosis in formalin-fixed tissue.

@article{Fukunaga2002SensitivityOA,
  title={Sensitivity of acid-fast staining for Mycobacterium tuberculosis in formalin-fixed tissue.},
  author={Hajime Fukunaga and Tomoyuki Murakami and Toshikazu Gondo and Kazuo Sugi and Tokuhiro Ishihara},
  journal={American journal of respiratory and critical care medicine},
  year={2002},
  volume={166 7},
  pages={
          994-7
        }
}
Microscopic examination of tissue sections of mycobacterial lesions frequently results in few or no bacilli seen, even if the lesions appear active histologically. This might be due to the effects of the fixative fluid and/or organic solvent, both of which are conventionally used to make tissue sections for histopathology, on the acid-fast staining of bacteria. The present study was performed to examine how formalin and xylene lower the sensitivity of acid-fast staining for Mycobacterium… CONTINUE READING
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