Sensitivity of PCR targeting Mycobacterium ulcerans by use of fine-needle aspirates for diagnosis of Buruli ulcer.

@article{Phillips2009SensitivityOP,
  title={Sensitivity of PCR targeting Mycobacterium ulcerans by use of fine-needle aspirates for diagnosis of Buruli ulcer.},
  author={R O Phillips and Fred Stephen Sarfo and F Osei-Sarpong and Asiedu Boateng and Ishmael Tetteh and A Lartey and E Adentwe and William Opare and Kingsley Bampoe Asiedu and Mark H. Wansbrough-Jones},
  journal={Journal of clinical microbiology},
  year={2009},
  volume={47 4},
  pages={
          924-6
        }
}

• R O Phillips, Fred Stephen Sarfo, +7 authors Mark H. Wansbrough-Jones
• Published in
  Journal of clinical…
  2009
• DOI:10.1128/JCM.01842-08

In a previous study, we reported that the sensitivity of PCR targeting the IS2404 insertion sequence of Mycobacterium ulcerans was 98% when it was applied to 4-mm punch biopsy samples of Buruli lesions. Fine-needle aspiration (FNA) is a less traumatic sampling technique for nonulcerated lesions, and we have studied the sensitivity of PCR using FNA samples. Fine-needle aspirates were taken with a 21-gauge needle from 43 patients diagnosed clinically with M. ulcerans disease. Four-millimeter… CONTINUE READING

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