Sensitivity of PCR targeting Mycobacterium ulcerans by use of fine-needle aspirates for diagnosis of Buruli ulcer.

@article{Phillips2009SensitivityOP,
  title={Sensitivity of PCR targeting Mycobacterium ulcerans by use of fine-needle aspirates for diagnosis of Buruli ulcer.},
  author={R O Phillips and Fred Stephen Sarfo and F Osei-Sarpong and Asiedu Boateng and Ishmael Tetteh and A Lartey and E Adentwe and William Opare and Kingsley Bampoe Asiedu and Mark H. Wansbrough-Jones},
  journal={Journal of clinical microbiology},
  year={2009},
  volume={47 4},
  pages={
          924-6
        }
}
In a previous study, we reported that the sensitivity of PCR targeting the IS2404 insertion sequence of Mycobacterium ulcerans was 98% when it was applied to 4-mm punch biopsy samples of Buruli lesions. Fine-needle aspiration (FNA) is a less traumatic sampling technique for nonulcerated lesions, and we have studied the sensitivity of PCR using FNA samples. Fine-needle aspirates were taken with a 21-gauge needle from 43 patients diagnosed clinically with M. ulcerans disease. Four-millimeter… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 28 CITATIONS

Buruli Ulcer in Liberia, 2012

  • Emerging infectious diseases
  • 2014
VIEW 3 EXCERPTS
CITES METHODS

References

Publications referenced by this paper.
SHOWING 1-10 OF 16 REFERENCES

Fine-needle aspiration in the diagnosis of head and neck lesions: a review and discussion of problems in differential diagnosis

J. Lester, M. D. Layfield
  • Diagn. Cytopathol
  • 2007
VIEW 1 EXCERPT

Buruli Ulcer Recurrence, Benin

  • Emerging infectious diseases
  • 2005
VIEW 2 EXCERPTS

Buruli ulcer : management of Mycobacterium ulcerans disease . A manual for health care providers

A. Chauty, M. F. Ardant, +6 authors J. Grosset
  • 2001

Buruli ulcer: diagnosis of Mycobacterium ulcerans disease

F. Portaels, P. Johnson, W. E. Meyers
  • World Heath Organization,
  • 2001

Buruli ulcer: management of Mycobacterium ulcerans disease. A manual for health care providers. World Health Organization, Geneva, Switzerland. http://www.who.int/gtb-buruli/publications /index.html

J. Buntine, K. E. Crofts
  • 2001
VIEW 1 EXCERPT