Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.

  title={Sensitivity-enhanced solid-state NMR detection of expansin's target in plant cell walls.},
  author={Tuo Wang and Yong Bum Park and Marc A. Caporini and M{\'e}lanie Rosay and Linghao Zhong and Daniel J Cosgrove and Mei Hong},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={110 41},
Structure determination of protein binding to noncrystalline macromolecular assemblies such as plant cell walls (CWs) poses a significant structural biology challenge. CWs are loosened during growth by expansin proteins, which weaken the noncovalent network formed by cellulose, hemicellulose, and pectins, but the CW target of expansins has remained elusive because of the minute amount of the protein required for activity and the complex nature of the CW. Using solid-state NMR spectroscopy… CONTINUE READING
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