Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories.

@article{Noordhoek1994SensitivityAS,
  title={Sensitivity and specificity of PCR for detection of Mycobacterium tuberculosis: a blind comparison study among seven laboratories.},
  author={Gerda T. Noordhoek and A. J. Kolk and Gunnar Bjune and D. Catty and Jeremy W. Dale and Paul E. M. Fine and Peter Godfrey-Faussett and Sang Nae Cho and Thomas Shinnick and Stefan B. Svenson},
  journal={Journal of clinical microbiology},
  year={1994},
  volume={32 2},
  pages={277-84}
}
PCR is, in principle, a simple and rapid test for use in the detection of Mycobacterium tuberculosis. However, virtually no data are available on the reliability and reproducibility of the method. In order to assess the validity of PCR for the detection of mycobacteria in clinical samples, seven laboratories participated in a blinded study of 200 sputum, saliva, and water samples containing either known numbers of Mycobacterium bovis BCG cells or no added organisms. Each laboratory used its own… CONTINUE READING