Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR.

@article{Matsuda2007SensitiveQD,
  title={Sensitive quantitative detection of commensal bacteria by rRNA-targeted reverse transcription-PCR.},
  author={Kazunori Matsuda and Hirokazu Tsuji and Takashi Asahara and Yukiko Kado and Koji Nomoto},
  journal={Applied and environmental microbiology},
  year={2007},
  volume={73 1},
  pages={
          32-9
        }
}
A sensitive rRNA-targeted reverse transcription-quantitative PCR (RT-qPCR) method was developed for exact and sensitive enumeration of subdominant bacterial populations. Using group- or species-specific primers for 16S or 23S rRNA, analytical curves were constructed for Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, Clostridium perfringens, and Pseudomonas aeruginosa, and the threshold cycle value was found to be linear up to an RNA amount of 10(-3) cell per RT-PCR. The number… CONTINUE READING

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