Sensitive quantitation of nitric oxide by EPR spectroscopy.

  title={Sensitive quantitation of nitric oxide by EPR spectroscopy.},
  author={Koichiro Tsuchiya and Mitsuo Takasugi and Kazuo Minakuchi and Kenji Fukuzawa},
  journal={Free radical biology & medicine},
  volume={21 5},
A recent method for NO detection is electron paramagnetic resonance (EPR) with ferrous and mononitrosyl dithiocarbamate (Fe2+ (DETC)2) for spin trapping [Menon, N.K., et al., J.Mol. Cell Cardiol., 23:389; 1991]. However, by this technique, we failed to detect the spectrum of the NOFe2+ (DETC)2 complex in biological systems because of the low solubility of Fe2+ (DETC)2 and rapid oxidation of NOFe2+ (DETC)2 complex. To overcome these problems, we modified this method by adding albumin to… CONTINUE READING


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