Sensitive, practical bioassay of thyrotropin, with use of FRTL-5 thyroid cells and magnetizable solid-phase-bound antibodies.

Abstract

We have developed a new method for assessing the bioactivity of thyrotropin (TSH) in human serum by measuring cAMP production in FRTL-5 thyroid cells as an index of stimulation. To eliminate serum inhibitors of the cAMP response to TSH, we purified samples by mixing them with anti-TSH antibodies coupled to magnetizable particles. This method of… (More)

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Cite this paper

@article{Tokuda1988SensitivePB, title={Sensitive, practical bioassay of thyrotropin, with use of FRTL-5 thyroid cells and magnetizable solid-phase-bound antibodies.}, author={Yutaka Tokuda and Kanji Kasagi and Yasuhiko Iida and Hiroto Hatabu and Akihisa Hidaka and Takashi Misaki and Jun Konishi}, journal={Clinical chemistry}, year={1988}, volume={34 11}, pages={2360-4} }