Sensitive methods to study human apolipoprotein B metabolism using stable isotope-labeled amino acids.

@article{Demant1996SensitiveMT,
  title={Sensitive methods to study human apolipoprotein B metabolism using stable isotope-labeled amino acids.},
  author={Th Demant and Christopher J. Packard and Hans Demmelmair and Philip Stewart and Andrea Bedynek and Dorothy K Bedford and Dietrich Seidel and James Michael Shepherd},
  journal={The American journal of physiology},
  year={1996},
  volume={270 6 Pt 1},
  pages={E1022-36}
}
The objective of the study was to develop a sensitive method using stable isotope-labeled tracers that would permit the determination of apolipoprotein B (apoB) metabolism in very low-density lipoprotein subfractions (VLDL1, Sf 60-400; VLDL2, Sf 20-60), intermediate-density lipoprotein (IDL, Sf 12-20), and low-density lipoprotein (LDL, Sf 0-12). Six normolipidemic subjects were given trideuterated leucine, and its clearance from plasma and appearance in the four apoB-containing lipoprotein… CONTINUE READING