Sensitive ligand-based protein quantification using immuno-PCR: A critical review of single-probe and proximity ligation assays.

Abstract

Quantitative PCR (qPCR) of reverse-transcribed mRNA has revolutionized gene expression analyses. qPCR analysis is based on the prevalent assumption that mRNA transcript numbers provide an adequate measure of specific biomarker expression. However, taking the complexity of protein turnover into account, there is a need to correlate qPCR-derived… (More)

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