Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography: detection of single-copy human papillomavirus.

@article{Zehbe1997SensitiveIS,
  title={Sensitive in situ hybridization with catalyzed reporter deposition, streptavidin-Nanogold, and silver acetate autometallography: detection of single-copy human papillomavirus.},
  author={Ingeborg Zehbe and Gerhard Hacker and Hung-Ju Su and Cornelia Hauser-kronberger and James F. Hainfeld and Raymond Tubbs},
  journal={The American journal of pathology},
  year={1997},
  volume={150 5},
  pages={1553-61}
}
The usefulness of standard in situ hybridization for viral nucleic acid detection is occasionally limited by its sensitivity limit of 10 to 50 copies per cell. A modified version of the recently described signal amplification method, catalyzed reporter deposition (CARD), and its application to formalin-fixed cells and tissue sections is presented. Deposition of the reporter is facilitated by using horseradish peroxidase catalyzing the deposition of biotinylated tyramide on the location of the… CONTINUE READING