Sensitive enantiomer-specific high-performance liquid chromatographic analysis of methamphetamine and amphetamine from serum using precolumn fluorescent derivatization.

@article{Hutchaleelaha1994SensitiveEH,
  title={Sensitive enantiomer-specific high-performance liquid chromatographic analysis of methamphetamine and amphetamine from serum using precolumn fluorescent derivatization.},
  author={Athiwat Hutchaleelaha and Adam G Walters and H-H. Sherry Chow and Michael Mayersohn},
  journal={Journal of chromatography. B, Biomedical applications},
  year={1994},
  volume={658 1},
  pages={103-12}
}
In order to study the stereoselective disposition of methamphetamine (MAP), a widely abused drug, we have developed a sensitive HPLC assay to separate and quantitate the enantiomers of MAP and amphetamine (AP) in rat serum. Serum samples to which was added aniline sulfate (internal standard) were alkalized with 0.02 M carbonate buffer (pH 10.6) and extracted with ethyl acetate. Following back extraction with hydrochloric acid, neutralization, and reconstitution, the sample was derivatized with… CONTINUE READING