Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR.

@article{Adams2014SensitiveDO,
  title={Sensitive diagnosis of cutaneous leishmaniasis by lesion swab sampling coupled to qPCR.},
  author={Emily Rebecca Adams and Mar{\'i}a Adelaida G{\'o}mez and Laura Scheske and Ruby Rios and Ricardo M{\'a}rquez and Alexandra Cossio and Audrey Albertini and Henk D. H. F. Schallig and Nancy Gore Saravia},
  journal={Parasitology},
  year={2014},
  volume={141 14},
  pages={1891-7}
}
Variation in clinical accuracy of molecular diagnostic methods for cutaneous leishmaniasis (CL) is commonly observed depending on the sample source, the method of DNA recovery and the molecular test. Few attempts have been made to compare these variables. Two swab and aspirate samples from lesions of patients with suspected CL (n = 105) were evaluated alongside standard diagnosis by microscopic detection of amastigotes or culture of parasites from lesion material. Three DNA extraction methods… CONTINUE READING