Sensitive determination of pheomelanin after 5-carboxyfluorescein succinimidyl ester precapillary derivatization and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.

Abstract

The suitability of micellar electrokinetic capillary chromatography (MEKC) with laser-induced fluorescence (LIF) detection for the determination of pheomelanin in biological materials has been investigated. 5-Carboxyfluorescein succinimidyl ester was chosen as the labeling reagent to precapillary derivatize the two marker aminohydroxyphenylalanine (AHP… (More)

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@article{Zhang2008SensitiveDO, title={Sensitive determination of pheomelanin after 5-carboxyfluorescein succinimidyl ester precapillary derivatization and micellar electrokinetic capillary chromatography with laser-induced fluorescence detection.}, author={Xiao-ling Zhang and Qiao Yang and Bing-hua Jiao and Jun Dai and Jun-xiang Zhang and Wu-zhong Ni}, journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences}, year={2008}, volume={861 1}, pages={136-9} }