Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies.

@article{Deelder1989SensitiveDO,
  title={Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies.},
  author={Andr{\'e} M. Deelder and Nicolaas de Jonge and Otto C Boerman and Yvonne E. Filli{\'e} and G W Hilberath and J. Peter Rotmans and M J Gerritse and D W Schut},
  journal={The American journal of tropical medicine and hygiene},
  year={1989},
  volume={40 3},
  pages={268-72}
}
From a panel of mouse monoclonal antibodies reactive with a repeating epitope of the schistosome circulating anodic antigen, an IgG1 monoclonal antibody was selected. This monoclonal antibody was applied in a sandwich enzyme-linked immunosorbent assay as capture antibody and as alkaline phosphatase labeled conjugate. This assay allowed a sensitive quantitation of circulating anodic antigen in serum samples of infected individuals, detecting less than 1 ng antigen/ml serum. In Schistosoma… CONTINUE READING
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