Sensitive determination of BRAF copy number in clinical samples by pyrosequencing.

@article{Setty2011SensitiveDO,
  title={Sensitive determination of BRAF copy number in clinical samples by pyrosequencing.},
  author={Prashanth Haranahalli Chandrappa Setty and Marco Gessi and Andreas Waha and Jennifer Hammes and Osman el-Maarri and Torsten Pietsch},
  journal={Diagnostic molecular pathology : the American journal of surgical pathology, part B},
  year={2011},
  volume={20 3},
  pages={148-57}
}
Pilocytic astrocytoma is the most frequently occurring brain tumor during childhood. It is classified as grade I by the World Health Organization and may rarely evolve into higher-grade tumors. Frequent genetic abnormalities documented in astrocytomas in children are gains on chromosomal arm 7q. Duplications at 7q34 lead to a fusion between genes KIAA1549 and BRAF resulting in constitutive activation of the BRAF kinase. The BRAF gene is located on chromosome 7q34 and a pseudogene has been… CONTINUE READING