Sensitive detection of two IgG Fc receptors of mouse macrophages by chemiluminescence analysis.

@article{Majima1989SensitiveDO,
  title={Sensitive detection of two IgG Fc receptors of mouse macrophages by chemiluminescence analysis.},
  author={Takashi Majima and Kyogo Itoh and J Yatsu and Osamu Yoshie and Nobuhiro Ishida},
  journal={Immunopharmacology and immunotoxicology},
  year={1989},
  volume={11 2-3},
  pages={289-307}
}
Luminol-enhanced chemiluminescence assay was used to detect the surface expression and the consequent activation of receptors (FcRI and FcRII) of murine macrophages (M phi s). When murine IgG2a was used for the specific detection of FcRI and IgG2b for FcRII, a newly established procedure enabled us to detect the activation of each receptor with as few as 3 X 10(5) M phi s. Briefly, TNP-SRBC coated with monoclonal IgG2a or IgG2b antibodies directed to TNP (sensitized SRBC) were used as reagent… CONTINUE READING