Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding

@article{Saboro2001SensitiveDO,
  title={Sensitive detection of pathological prion protein by cyclic amplification of protein misfolding},
  author={Gabriela P. Sabor{\'i}o and Bruno Permanne and Claudio Soto},
  journal={Nature},
  year={2001},
  volume={411},
  pages={810-813}
}
Prions are the infectious agents responsible for transmissible spongiform encephalopathies. The principal component of prions is the glycoprotein PrPSc, which is a conformationally modified isoform of a normal cell-surface protein called PrPC (ref. 1). During the time between infection and the appearance of the clinical symptoms, minute amounts of PrPSc replicate by conversion of host PrPC, generating large amounts of PrPSc aggregates in the brains of diseased individuals. We aimed to reproduce… Expand

Paper Mentions

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TLDR
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TLDR
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TLDR
This chapter describes a method for amplifying PrP(Sc) molecules from hamster prions in vitro using purified substrates, and specific protocols for substrate preparation, reaction mixture, and product detection are explained. Expand
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