Sensitive detection of grapevine virus A, B, or leafroll-associated III from viruliferous mealybugs and infected tissue by cDNA amplification.

@article{Minafra1994SensitiveDO,
  title={Sensitive detection of grapevine virus A, B, or leafroll-associated III from viruliferous mealybugs and infected tissue by cDNA amplification.},
  author={Angelantonio Minafra and Ahmed Hadidi},
  journal={Journal of virological methods},
  year={1994},
  volume={47 1-2},
  pages={175-87}
}
DNA primers specific for grapevine virus A (GVA), grapevine virus B (GVB) or grapevine leafroll-associated virus III (GLRaV-III) were constructed based on the nucleotide sequence of a segment of each viral genome. DNA primers were utilized for cDNA synthesis and polymerase chain reaction (PCR) amplification of a 430 bp fragment from extracts of GVA-infected grapevine tissue or viruliferous mealybugs and 450 bp and 340 bp DNA fragments from extracts of GVB and GLRaV-III-infected grapevine… CONTINUE READING

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