Sensitive detection of canine parvovirus DNA by the nested polymerase chain reaction.

@article{Hirasawa1994SensitiveDO,
  title={Sensitive detection of canine parvovirus DNA by the nested polymerase chain reaction.},
  author={Tsutomu Hirasawa and Takahiro Kaneshige and K Mikazuki},
  journal={Veterinary microbiology},
  year={1994},
  volume={41 1-2},
  pages={135-45}
}
A polymerase chain reaction (PCR) for the detection of canine parvovirus (CPV) was developed. To increase the sensitivity and specificity of the reaction, the nested PCR with a double-nested primer pair (inner primer pair) was designed. The sequences of the PCR primer pairs were selected from the conserved region in the CPV VP1/VP2 gene. The PCR with the outer or inner primer pair alone (single PCR) could detect 10 fg of viral replicative form (RF) DNA on agarose gel electrophoresis; whereas as… CONTINUE READING
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