Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads.

@article{Panhorst2005SensitiveBM,
  title={Sensitive bondforce measurements of ligand-receptor pairs with magnetic beads.},
  author={Michael Panhorst and P. H. J. van der Kamp and G{\"u}nter Reiss and Hubert Brueckl},
  journal={Biosensors & bioelectronics},
  year={2005},
  volume={20 8},
  pages={1685-9}
}
The bondforces between biotinylated surfaces and streptavidin or avidin coated beads are investigated by a magnetic field based manipulation system for magnetic microbeads. The magnetic field is generated by currents through a set of conducting lines, and its gradient exerts a force onto the magnetic beads. The force can be increased until the bond between the bead and the surface breaks. Consistent with other groups we found two conformations for both investigated bonds. The measured… CONTINUE READING