Sensitive assay for molluscan metallothionein induction based on ribonuclease protection and molecular titration of metallothionein and actin mRNAs.

Abstract

We developed a sensitive assay for oyster metallothionein (MT) mRNA based on ribonuclease protection and molecular titration. Deletion constructs derived from previously described MT cDNA and newly constructed actin cDNA clones were used to create templates for MT and actin riboprobe synthesis, respectively. MT induction was assessed by normalizing MT… (More)

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@article{Unger1993SensitiveAF, title={Sensitive assay for molluscan metallothionein induction based on ribonuclease protection and molecular titration of metallothionein and actin mRNAs.}, author={Markus Unger and Guri Roesijadi}, journal={Molecular marine biology and biotechnology}, year={1993}, volume={2 5}, pages={319-24} }