Sensitive assay for determining plasma tenofovir concentrations by LC/MS/MS.

  title={Sensitive assay for determining plasma tenofovir concentrations by LC/MS/MS.},
  author={Tom Delahunty and Lane R. Bushman and Courtney V Fletcher},
  journal={Journal of chromatography. B, Analytical technologies in the biomedical and life sciences},
  volume={830 1},
An LC/MS/MS assay for the determination of tenofovir (TNF) was developed and validated for use with the EDTA anticoagulated human plasma matrix. Heparin-treated plasma and serum matrices were also validated. After addition of adefovir as an internal standard, trifluoroacetic acid was used to produce a protein-free extract. Chromatographic separation was achieved with a Polar-RP Synergi, 2.0 mm x 150 mm, reversed-phase analytical column. The mobile phase was 3% acetonitrile/1% acetic acid, aq… CONTINUE READING

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