Sensitive and specific measurement of minimal residual disease in acute lymphoblastic leukemia.

@article{Morley2009SensitiveAS,
  title={Sensitive and specific measurement of minimal residual disease in acute lymphoblastic leukemia.},
  author={Alexander Morley and Sue Latham and Michael J. Brisco and Pamela J Sykes and Rosemary Sutton and Elizabeth Hughes and Vicki Wilczek and Bradley J Budgen and Katrina van Zanten and Bryone J Kuss and Nicola C. Venn and Murray D. Norris and Catherine M Crock and Colin Storey and Tamas R Revesz and Keith Waters},
  journal={The Journal of molecular diagnostics : JMD},
  year={2009},
  volume={11 3},
  pages={201-10}
}
A sensitive and specific quantitative real-time polymerase chain reaction method, involving three rounds of amplification with two allele-specific oligonucleotide primers directed against an rearrangement, was developed to quantify minimal residual disease (MRD) in B-lineage acute lymphoblastic leukemia (ALL). For a single sample containing 10 microg of good quality DNA, MRD was quantifiable down to approximately 10(-6), which is at least 1 log more sensitive than current methods. Nonspecific… CONTINUE READING