Sensitive and selective magnetoimmunosensing platform for determination of the food allergen Ara h 1.

@article{Montiel2015SensitiveAS,
  title={Sensitive and selective magnetoimmunosensing platform for determination of the food allergen Ara h 1.},
  author={V{\'i}ctor Ruiz-Valdepe{\~n}as Montiel and Susana Campuzano and Alessandro Pellican{\`o} and Rebeca M. Torrente-Rodr{\'i}guez and A Julio Reviejo and Mar{\'i}a Cosio and Jos{\'e} Manuel Pingarr{\'o}n},
  journal={Analytica chimica acta},
  year={2015},
  volume={880},
  pages={52-9}
}
A highly sensitive disposable amperometric immunosensor based on the use of magnetic beads (MBs) is described for determination of Ara h 1, the major peanut allergen, in only 2h. The approach uses a sandwich configuration involving selective capture and biotinylated detector antibodies and carboxylic acid-modified MBs (HOOC-MBs). The MBs bearing the immunoconjugates are captured by a magnet placed under the surface of a disposable screen-printed carbon electrode (SPCE) and the affinity… CONTINUE READING