Sensitive and reliable detection of grapevine fanleaf virus in a single Xiphinema index nematode vector.

@article{Demangeat2004SensitiveAR,
  title={Sensitive and reliable detection of grapevine fanleaf virus in a single Xiphinema index nematode vector.},
  author={G{\'e}rard Demangeat and V{\'e}ronique Komar and Pascal Cornuet and Daniel Esmenjaud and Marc Fuchs},
  journal={Journal of virological methods},
  year={2004},
  volume={122 1},
  pages={79-86}
}
Grapevine fanleaf virus (GFLV) is specifically transmitted from plant to plant by the ectoparasitic nematode Xiphinema index. A sensitive and reliable procedure was developed to readily detect GFLV in a single viruliferous X. index, regardless of the nematode origin, i.e. greenhouse rearings or vineyard soils. The assay is based on bead milling to disrupt nematodes extracted from soil samples, solid-phase extraction of total nematode RNAs, and amplification of a 555bp fragment of the coat… CONTINUE READING

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