Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction.

@article{Jiang2004SensitiveAQ,
  title={Sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction.},
  author={Shih Sheng Jiang and T H Chen and J Y Yang and Chao A. Hsiung and I Fan Su and Ying-lan Liu and Po-Cheng Chen and J P Juang},
  journal={Clinical infectious diseases : an official publication of the Infectious Diseases Society of America},
  year={2004},
  volume={38 2},
  pages={293-6}
}
A quantitative, real-time, nested polymerase chain reaction (PCR) method, combining the high sensitivity of nested PCR with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (SARS) coronavirus. Forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase PCR (RT-PCR) performed in parallel. Of the 17 positive results, 2 identified by… CONTINUE READING