Sensitive and highly specific quantitative real-time PCR and ELISA for recording a potential transfer of novel DNA and Cry1Ab protein from feed into bovine milk

@article{Guertler2009SensitiveAH,
  title={Sensitive and highly specific quantitative real-time PCR and ELISA for recording a potential transfer of novel DNA and Cry1Ab protein from feed into bovine milk},
  author={Patrick Guertler and Vijay Paul and Christiane Albrecht and Heinrich H. D. Meyer},
  journal={Analytical and Bioanalytical Chemistry},
  year={2009},
  volume={393},
  pages={1629-1638}
}
AbstractTo address food safety concerns of the public regarding the potential transfer of recombinant DNA (cry1Ab) and protein (Cry1Ab) into the milk of cows fed genetically modified maize (MON810), a highly specific and sensitive quantitative real-time PCR (qPCR) and an ELISA were developed for monitoring suspicious presence of novel DNA and Cry1Ab protein in bovine milk. The developed assays were validated according to the assay validation criteria specified in the European Commission… 
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A real-time immuno-PCR assay for the detection of transgenic Cry1Ab protein

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  • Biology
    European Food Research and Technology
  • 2011
The real-time immuno-PCR (IPCR) assay was developed for the detection and quantification of Cry1Ab protein and showed high sensitivity with minimum detection limit and found to be 10 times more sensitive than sandwich ELISA.

Development of ELISA for the detection of transgenic vegetative insecticidal protein in GM crops/produce.

  • R. Kumar
  • Biology
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  • 2012
The ELISA assay developed to support the labelling of vegetative insecticidal protein (Vip3A)-based GM produce was specific, sensitive and reproducible, which can be helpful to detect and track down the spread of unapproved and intentionally/unintentionally released GM produce harbouring Vip protein.

Establishment of a sandwich enzyme-linked immunosorbent assay for specific detection of Bacillus thuringiensis (Bt) Cry1Ab toxin utilizing a monoclonal antibody produced with a novel hapten designed with molecular model

A novel hapten polypeptide was synthesized and conjugated to keyhole limpet hemocyanin (KLH) and a monoclonal antibody named 2C12, showing high affinity to Cry1Ab and having no cross reaction with Cry1Ac, was produced, providing a new approach for the production of high specific antibody.

A novel sandwich ELISA system using rabbit monoclonal and polyclonal antibodies for rapid detection of Bt-Cry1Ac protein

The LOD and the linear detection range of the proposed ELISA are more satisfactory than other ELISA systems using an anti-Cry1Ac PcAb or two Cry1Ac-specific McAb for detection of the target protein and the research provides an effective instrument for detecting Cry1ac protein in GM crops and their derivatives.

Development of monoclonal antibody-based sensitive ELISA for the determination of Cry1Ie protein in transgenic plant

The results in this study suggested that the established ELISA method is effective for detecting Cry1Ie protein in transgenic plants.

Established a new double antibodies sandwich enzyme-linked immunosorbent assay for detecting Bacillus thuringiensis (Bt) Cry1Ab toxin based single-chain variable fragments from a naïve mouse phage displayed library.

Potential of cross-priming amplification and DNA-based lateral-flow strip biosensor for rapid on-site GMO screening

An on-site method for rapid detection of cauliflower mosaic virus promoter (CaMV 35S), a common element present in most GMO, using cross-priming amplification (CPA) technology, which has great commercial prospects for rapid GMO screening of high-consumption food and agriculture products.

Comparison of quantitative PCR and culture-based methods for evaluating dispersal of Bacillus thuringiensis endospores at a bioterrorism hoax crime scene.

Phage-Mediated Competitive Chemiluminescent Immunoassay for Detecting Cry1Ab Toxin by Using an Anti-Idiotypic Camel Nanobody.

It is demonstrated that the competitive format based on phage-displayed anti-idiotypic nanobodies can provide an alternative strategy for Cry toxin detection, with negligible cross-reactivity for other Cry toxins.

Long-term feeding of genetically modified maize (MON810) - Metabolism of recombinant DNA and the novel protein in the dairy cow

Only in feces of the transgenic fed group, the Cry1Ab protein was found, and this protein underlies a faster degradation than other proteins in feed, suggesting that it underlies the faster degradation of the cry1Ab DNA and the novel protein.

References

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