Sensitive and highly specific quantitative real-time PCR and ELISA for recording a potential transfer of novel DNA and Cry1Ab protein from feed into bovine milk

@article{Guertler2009SensitiveAH,
  title={Sensitive and highly specific quantitative real-time PCR and ELISA for recording a potential transfer of novel DNA and Cry1Ab protein from feed into bovine milk},
  author={P. Guertler and V. Paul and C. Albrecht and H. Meyer},
  journal={Analytical and Bioanalytical Chemistry},
  year={2009},
  volume={393},
  pages={1629-1638}
}
  • P. Guertler, V. Paul, +1 author H. Meyer
  • Published 2009
  • Biology, Medicine
  • Analytical and Bioanalytical Chemistry
  • AbstractTo address food safety concerns of the public regarding the potential transfer of recombinant DNA (cry1Ab) and protein (Cry1Ab) into the milk of cows fed genetically modified maize (MON810), a highly specific and sensitive quantitative real-time PCR (qPCR) and an ELISA were developed for monitoring suspicious presence of novel DNA and Cry1Ab protein in bovine milk. The developed assays were validated according to the assay validation criteria specified in the European Commission… CONTINUE READING
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