Sensitive analysis of oxidative DNA damage in mammalian cells: use of the bacterial Fpg protein in combination with alkaline unwinding.

@article{Hartwig1996SensitiveAO,
  title={Sensitive analysis of oxidative DNA damage in mammalian cells: use of the bacterial Fpg protein in combination with alkaline unwinding.},
  author={Andrea Hartwig and H Dally and Regina Schlepegrell},
  journal={Toxicology letters},
  year={1996},
  volume={88 1-3},
  pages={85-90}
}
The measurement of oxidative DNA base modifications by different methods has received special attention in recent years. Here we describe a procedure to quantify DNA lesions recognized by the bacterial formamido-pyrimidine-DNA glycosylases (Fpg protein). These include 7,8-dihydro-8-oxoguanine (8-hydroxyguanine) as well as some other forms of imidazole ring-opened purines, which are converted into abasic sites and subsequently into DNA single-strand breaks by the associated endonuclease activity… CONTINUE READING

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 18 extracted citations

In vitro and in vivo genotoxicity investigations of differently sized amorphous SiO2 nanomaterials.

Mutation research. Genetic toxicology and environmental mutagenesis • 2015
View 1 Excerpt

8-hydroxy-2' -deoxyguanosine (8-OHdG): A critical biomarker of oxidative stress and carcinogenesis.

Journal of environmental science and health. Part C, Environmental carcinogenesis & ecotoxicology reviews • 2009

Similar Papers

Loading similar papers…