Sensitive Impedimetric Immunoassay of Japanese Encephalitis Virus Based on Enzyme Biocatalyzed Precipitation on a Gold Nanoparticle-modified Screen-printed Carbon Electrode.

Abstract

A sensitive and disposable electrochemical impedance biosensor to detect Japanese encephalitis virus (JEV) was developed based on a gold nanoparticle (AuNP)-modified screen-printed carbon electrode (SPCE). A biosensor was fabricated through covalent grafting of a mixed self-assembled monolayer on AuNPs with a specific antibody. To detect JEV and achieve signal amplification, the horseradish peroxidase (HRP)-labeled second antibody was linked to the biosensor through a sandwich immunity reaction. HRP was used to catalyze 4-chloro-1-naphthol oxidation to produce an insoluble precipitate, which introduced a barrier to electron transfer on the electrode. Electrochemical impedance spectroscopy (EIS) was used to monitor the precipitation on the electrode. The electron-transfer resistance (Ret) of the biosensor was directly correlated with the concentration of JEV in the solution. Under optimal conditions, the method generated a linear response range between 500 and 5 × 10(5) pfu mL(-1), and the detection limit was 167 pfu mL(-1). The biosensor exhibited good selectivity against other viruses.

Cite this paper

@article{Geng2016SensitiveII, title={Sensitive Impedimetric Immunoassay of Japanese Encephalitis Virus Based on Enzyme Biocatalyzed Precipitation on a Gold Nanoparticle-modified Screen-printed Carbon Electrode.}, author={Xiaohui Geng and Fanglin Zhang and Qiang Gao and Yingfeng Lei}, journal={Analytical sciences : the international journal of the Japan Society for Analytical Chemistry}, year={2016}, volume={32 10}, pages={1105-1109} }