Sensitive, semi-nested RT-PCR amplification of fusion gene sequences for the rapid detection and differentiation of Newcastle disease virus.

@article{Zhang2010SensitiveSR,
  title={Sensitive, semi-nested RT-PCR amplification of fusion gene sequences for the rapid detection and differentiation of Newcastle disease virus.},
  author={Lei Zhang and Zhiming Pan and Shizhong Geng and Xiang Chen and Shunling Hu and Huimo Liu and Yantao Wu and Xinan Jiao and Xiufan Liu},
  journal={Research in veterinary science},
  year={2010},
  volume={89 2},
  pages={282-9}
}
A rapid, sensitive and specific semi-nested RT-PCR was developed to detect and differentiate virulent and avirulent strains of Newcastle disease virus (NDV). For a total of 67 NDV strains, the results obtained from the semi-nested RT-PCR were consistent with those from nucleotide sequence analysis, plaque forming assays, mean death time (MDT) measurements and intracerebral pathogenicity index (ICPI). Furthermore, 13 class I NDV strains can be characterized by the semi-nested RT-PCR approach… CONTINUE READING