Sensing caspase 3 activity with quantum dot-fluorescent protein assemblies.

@article{Boeneman2009SensingC3,
  title={Sensing caspase 3 activity with quantum dot-fluorescent protein assemblies.},
  author={Kelly L. Boeneman and Baoliang Mei and Allison M. Dennis and Gang Bao and Jeffrey R. Deschamps and Hedi Mattoussi and Igor L. Medintz},
  journal={Journal of the American Chemical Society},
  year={2009},
  volume={131 11},
  pages={
          3828-9
        }
}
We demonstrate the use of a hybrid fluorescent protein semiconductor quantum dot (QD) sensor capable of specifically monitoring caspase 3 proteolytic activity. mCherry monomeric red fluorescent protein engineered to express an N-terminal caspase 3 cleavage site was ratiometrically self-assembled to the surface of QDs using metal-affinity coordination. The proximity of the fluorescent protein to the QD allows it to function as an efficient fluorescence resonance energy transfer acceptor… CONTINUE READING
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