Senescent fibroblasts induce moderate stress in lung epithelial cells in vitro.

Abstract

Epithelial-mesenchymal interactions contribute to functionality and integrity of the lung epithelium, which might change during ageing and associated cellular ageing. Therefore, we studied the effect of senescent versus pre-senescent lung fibroblasts (WI-38) on mitogenic and stress-protective factors in lung epithelial cells (H358). By use of conditioned medium, we found a growth promoting impact of fibroblasts compared with control medium from epithelial cells associated with activation of ERK1/2, Akt, p70S6K, and EGF receptor. Although senescent fibroblasts mediated similar growth stimulation compared with pre-senescent cells, we observed less protection against spontaneous mitochondrial dysfunction in vitro, higher production of reactive oxygen species and activation of copper/zinc superoxide dismutase. Moreover, senescent cells induced activation of caspase-3/7 in epithelial cells, which was associated with down-regulation of the caspase-inhibitory protein XIAP. In summary, senescent lung fibroblasts induce moderate stress in lung epithelial cells in vitro without affecting growth signaling.

Cite this paper

@article{Bartling2006SenescentFI, title={Senescent fibroblasts induce moderate stress in lung epithelial cells in vitro.}, author={Babett Bartling and Grit Rehbein and R. -E. Silber and Andreas Simm}, journal={Experimental gerontology}, year={2006}, volume={41 5}, pages={532-9} }