Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity.

  title={Semliki Forest virus mRNA capping enzyme requires association with anionic membrane phospholipids for activity.},
  author={Tero E. Ahola and Anja Lampio and Petri Auvinen and Leevi K{\"a}{\"a}ri{\"a}inen},
  journal={The EMBO journal},
  volume={18 11},
The replication complexes of all positive strand RNA viruses of eukaryotes are associated with membranes. In the case of Semliki Forest virus (SFV), the main determinant of membrane attachment seems to be the virus-encoded non-structural protein NSP1, the capping enzyme of the viral mRNAs, which has guanine-7-methyltransferase and guanylyltransferase activities. We show here that both enzymatic activities of SFV NSP1 are inactivated by detergents and reactivated by anionic phospholipids… CONTINUE READING


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