Semliki Forest virus 6K protein modifies membrane permeability after inducible expression in Escherichia coli cells.

@article{Sanz1994SemlikiFV,
  title={Semliki Forest virus 6K protein modifies membrane permeability after inducible expression in Escherichia coli cells.},
  author={Miguel Angel Sanz and Luis Perez and Luis Carrasco},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 16},
  pages={12106-10}
}
Semliki Forest virus encodes a small protein, known as 6K, that is associated with cellular membranes in the infected cells. This protein has been cloned and expressed in an inducible manner using pET vectors in Escherichia coli cells. Two different plasmids have been utilized; either the 6K gene is placed directly under the T7 promoter (pET3-6K) or the lac operator is located between the T7 promoter and the 6K gene (pET11-6K). In both systems, efficient synthesis of the 6K protein is achieved… CONTINUE READING

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