Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.

@article{Barbas1992SemisyntheticCA,
  title={Semisynthetic combinatorial antibody libraries: a chemical solution to the diversity problem.},
  author={Carlos F. Barbas and John D. Bain and Dick Hoekstra and Richard Allen Lerner},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 10},
  pages={4457-61}
}
The properties of naiveté and large diversity are considered to be essential starting features for combinatorial antibody libraries that eschew immunization by evolution in vitro. We have prepared large libraries with such properties by using random oligonucleotide synthesis, which has the potential to create approximately 10(20) complementarity-determining regions for antibody heavy chains. When combined with light chains and expressed on phage surfaces, high-affinity antibodies could be… CONTINUE READING

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