Semisynthesis of ubiquitylated proteins.

@article{McGinty2009SemisynthesisOU,
  title={Semisynthesis of ubiquitylated proteins.},
  author={Robert K. McGinty and Champak Chatterjee and Tom W Muir},
  journal={Methods in enzymology},
  year={2009},
  volume={462},
  pages={225-43}
}
Most, if not all, proteins are at one point or another posttranslationally modified so as to regulate their biological function. One of the most common protein modifications is ubiquitylation, in which the small protein ubiquitin is attached to a target protein in a multistep process involving dedicated ubiquitin ligases. Ubiquitylation is best known for its role in protein turnover. In this case, attachment of a polyubiquitin chain to a target protein leads to its eventual destruction by the… CONTINUE READING