Semisynthesis of biologically active glycoforms of the human cytokine interleukin 6.

@article{Reif2014SemisynthesisOB,
  title={Semisynthesis of biologically active glycoforms of the human cytokine interleukin 6.},
  author={A. Reif and S. Siebenhaar and A. Tr{\"o}ster and Marina Schm{\"a}lzlein and Carolin Lechner and Phanindra Velisetty and K. Gottwald and Claudia Poehner and I. Boos and Volker Schubert and S. Rose-John and C. Unverzagt},
  journal={Angewandte Chemie},
  year={2014},
  volume={53 45},
  pages={
          12125-31
        }
}
Human interleukin 6 (IL-6) is a potent cytokine with immunomodulatory properties. As the influence of N-glycosylation on the in vivo activities of IL-6 could not be elucidated so far, a semisynthesis of homogeneous glycoforms of IL-6 was established by sequential native chemical ligation. The four cysteines of IL-6 are convenient for ligations and require only the short synthetic glycopeptide 43-48. The Cys-peptide 49-183 could be obtained recombinantly by cleavage of a SUMO tag. The fragment 1… Expand
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