Semisynthesis of biologically active glycoforms of the human cytokine interleukin 6.

@article{Reif2014SemisynthesisOB,
  title={Semisynthesis of biologically active glycoforms of the human cytokine interleukin 6.},
  author={A. Reif and Sebastian Thomas Siebenhaar and Andrea Tr{\"o}ster and Marina Schm{\"a}lzlein and Carolin C. Lechner and Phanindra Velisetty and Karen Gottwald and Claudia Poehner and Irene Boos and Volker Schubert and Stefan Rose-John and Carlo Unverzagt},
  journal={Angewandte Chemie},
  year={2014},
  volume={53 45},
  pages={
          12125-31
        }
}
Human interleukin 6 (IL-6) is a potent cytokine with immunomodulatory properties. As the influence of N-glycosylation on the in vivo activities of IL-6 could not be elucidated so far, a semisynthesis of homogeneous glycoforms of IL-6 was established by sequential native chemical ligation. The four cysteines of IL-6 are convenient for ligations and require only the short synthetic glycopeptide 43-48. The Cys-peptide 49-183 could be obtained recombinantly by cleavage of a SUMO tag. The fragment 1… CONTINUE READING

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-8 OF 8 CITATIONS

Advances in Fmoc solid‐phase peptide synthesis

  • Journal of peptide science : an official publication of the European Peptide Society
  • 2016

References

Publications referenced by this paper.

Jaenicke in Protein Function (Ed.

R. Rudolph, G. Bçhm, R. H. Lilie
  • 1997