Semirational design of Jun-Fos coiled coils with increased affinity: Universal implications for leucine zipper prediction and design.

@article{Mason2006SemirationalDO,
  title={Semirational design of Jun-Fos coiled coils with increased affinity: Universal implications for leucine zipper prediction and design.},
  author={Jody M Mason and Mark A Schmitz and Kristian M. M{\"u}ller and Katja M. Arndt},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 24},
  pages={8989-94}
}
Activator protein-1 (AP-1) is a crucial transcription factor implicated in numerous cancers. For this reason, nine homologues of the AP-1 leucine zipper region have been characterized: Fos (c-Fos, FosB, Fra1, and Fra2), Jun (c-Jun, JunB, and JunD), and semirational library-designed winning peptides FosW and JunW. The latter two were designed to specifically target c-Fos or c-Jun. They have been identified by using protein-fragment complementation assays combined with growth competition. This… CONTINUE READING