Semirational Protein Engineering of CYP153AM.aq. -CPRBM3 for Efficient Terminal Hydroxylation of Short- to Long-Chain Fatty Acids.

@article{Notonier2016SemirationalPE,
  title={Semirational Protein Engineering of CYP153AM.aq. -CPRBM3 for Efficient Terminal Hydroxylation of Short- to Long-Chain Fatty Acids.},
  author={Sandra Notonier and Łukasz Gricman and J{\"u}rgen Pleiss and Bernhard Hauer},
  journal={Chembiochem : a European journal of chemical biology},
  year={2016},
  volume={17 16},
  pages={1550-7}
}
The regioselective terminal hydroxylation of alkanes and fatty acids is of great interest in a variety of industrial applications, such as in cosmetics, in fine chemicals, and in the fragrance industry. The chemically challenging activation and oxidation of non-activated C-H bonds can be achieved with cytochrome P450 enzymes. CYP153AM.aq. -CPRBM3 is an artificial fusion construct consisting of the heme domain from Marinobacter aquaeolei and the reductase domain of CYP102A1 from Bacillus… CONTINUE READING