Semirational Approach for Ultrahigh Poly(3-hydroxybutyrate) Accumulation in Escherichia coli by Combining One-Step Library Construction and High-Throughput Screening.

@article{Li2016SemirationalAF,
  title={Semirational Approach for Ultrahigh Poly(3-hydroxybutyrate) Accumulation in Escherichia coli by Combining One-Step Library Construction and High-Throughput Screening.},
  author={Teng Li and Jianwen Ye and Rui Shen and Yeqing Zong and Xuejin Zhao and Chunbo Lou and Guoqiang Chen},
  journal={ACS synthetic biology},
  year={2016},
  volume={5 11},
  pages={
          1308-1317
        }
}
As a product of a multistep enzymatic reaction, accumulation of poly(3-hydroxybutyrate) (PHB) in Escherichia coli (E. coli) can be achieved by overexpression of the PHB synthesis pathway from a native producer involving three genes phbC, phbA, and phbB. Pathway optimization by adjusting expression levels of the three genes can influence properties of the final product. Here, we reported a semirational approach for highly efficient PHB pathway optimization in E. coli based on a phbCAB operon… 
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