Semiquantitative detection by real-time PCR of Aspergillus fumigatus in bronchoalveolar lavage fluids and tissue biopsy specimens from patients with invasive aspergillosis.

@article{RantakokkoJalava2003SemiquantitativeDB,
  title={Semiquantitative detection by real-time PCR of Aspergillus fumigatus in bronchoalveolar lavage fluids and tissue biopsy specimens from patients with invasive aspergillosis.},
  author={Kaisu Rantakokko-Jalava and Sanna Laaksonen and Jouni Issakainen and Jukka Vauras and Jukka Nikoskelainen and Matti K. Viljanen and Juha Salonen},
  journal={Journal of clinical microbiology},
  year={2003},
  volume={41 9},
  pages={4304-11}
}
A real-time PCR method was developed and used to detect Aspergillus fumigatus mitochondrial DNA (mtDNA) in bronchoalveolar lavage (BAL) fluids and tissue biopsy specimens. The analytical sensitivity of the assay was one A. fumigatus conidium per reaction, and the assay was linear at least over 4 orders of magnitude above the detection limit. BAL fluids from 66 immunocompromised patients at risk of invasive pulmonary aspergillosis (IPA) and 33 immunocompetent controls and tissue biopsy specimens… CONTINUE READING