Seminar Topics

  title={Seminar Topics},
  author={Ho Mc and M{\'e}n{\'e}tret Jf and H Tsuruta and Allen Kn and Isom Dg and Casta{\~n}eda Ca and Cannon Br and Velu Pd and Enrique Garc{\'i}a-Moreno},
Acetoacetate decarboxylase (AADase) has long been cited as the prototypical example of the marked shifts in the pK(a) values of ionizable groups that can occur in an enzyme active site. In 1966, it was hypothesized that in AADase the origin of the large pK(a) perturbation (‐4.5 log units) observed in the nucleophilic Lys 115 results from the proximity of Lys 116, marking the first proposal of microenvironment effects in enzymology. The electrostatic perturbation hypothesis… CONTINUE READING