Semienclosed Tube Cultures of Bean Plants (Phaseolus vulgaris L.) for Enumeration of Rhizobium phaseoli by the Most-Probable-Number Technique.

@article{Araujo1986SemienclosedTC,
  title={Semienclosed Tube Cultures of Bean Plants (Phaseolus vulgaris L.) for Enumeration of Rhizobium phaseoli by the Most-Probable-Number Technique.},
  author={Rina Araujo and J Maya-Flores and D Barnes-McConnell and Chihiro Yokoyama and Frank B. Dazzo and Fredrick Allen Bliss},
  journal={Applied and environmental microbiology},
  year={1986},
  volume={52 4},
  pages={954-6}
}
The semienclosed tube culture technique of Gibson was modified to permit growth of common bean (Phaseolus vulgaris L.) roots in humid air, enabling enumeration of the homologous (nodule forming) symbiont, Rhizobium phaseoli, by the most-probable-number plant infection method. A bean genotype with improved nodulation characteristics was used as the plant host. This method of enumeration was accurate when tubes were scored 3 weeks after inoculation with several R. phaseoli strains diluted from… CONTINUE READING

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