Semiconductor-based DNA sequencing of histone modification states


The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.

DOI: 10.1038/ncomms3672

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@inproceedings{Cheng2013SemiconductorbasedDS, title={Semiconductor-based DNA sequencing of histone modification states}, author={Christine S. Cheng and Kunal Rai and Manuel Garber and Andrew Hollinger and Dana Robbins and Scott A. Anderson and Alyssa Macbeth and Austin Tzou and Mauricio O. Carneiro and Raktima Raychowdhury and Carsten Russ and Nir Hacohen and Jeffrey E Gershenwald and Niall J. Lennon and Chad Nusbaum and Lynda Chin and Aviv Regev and Ido Amit}, booktitle={Nature communications}, year={2013} }