Semiautomated computer-assisted image analysis to quantify 3,3'-diaminobenzidine tetrahydrochloride-immunostained small tissues.

@article{Leal2006SemiautomatedCI,
  title={Semiautomated computer-assisted image analysis to quantify 3,3'-diaminobenzidine tetrahydrochloride-immunostained small tissues.},
  author={Sandra M Leal and Carmen Simone Grilo Diniz and Carlos S{\'a} and Jorge Gonçalves and Ana Sofia Prior Soares and Carolina Rocha-Pereira and Paula Fresco},
  journal={Analytical biochemistry},
  year={2006},
  volume={357 1},
  pages={
          137-43
        }
}
This work aimed to develop a technique to measure stained areas in images from sample tissue sections, namely when the structure of interest does not fill the entire image field of the microscope. We propose a semiautomated computer-assisted image analysis (SACAIA) method in which brightfield color images of 3,3'-diaminobenzidene tetrahydrochloride (DAB)-stained antigens are converted to their blue component and boundaries are delineated to extract the object of interest. The number of pixels… CONTINUE READING
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