Semi-loose seal Neurobiotin electroporation for combined structural and functional analysis of neurons

Abstract

Intracellular sharp-electrode, whole-cell patch clamp and juxtacellular labeling methods have previously been developed for combined analysis of neuronal structure and function. We describe a novel electroporation technique for labeling neurons with Neurobiotin, using patch electrodes in a semi-loose seal configuration (R = 100–300 MΩ) with very small… (More)
DOI: 10.1007/s00424-008-0539-9

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