Semi-automatic determination of cell surface areas used in systems biology.

@article{Morath2013SemiautomaticDO,
  title={Semi-automatic determination of cell surface areas used in systems biology.},
  author={Volker Morath and Margret Keuper and Marta Rodriguez-Franco and Sumit Deswal and Gina J. Fiala and Britta Blumenthal and Daniel Kaschek and Jens Timmer and Gunther Neuhaus and Stephan Ehl and Olaf Ronneberger and Wolfgang W. Schamel},
  journal={Frontiers in bioscience},
  year={2013},
  volume={5},
  pages={
          533-45
        }
}
Quantitative biology requires high precision measurement of cellular parameters such as surface areas or volumes. Here, we have developed an integrated approach in which the data from 3D confocal microscopy and 2D high-resolution transmission electron microscopy were combined. The volumes and diameters of the cells within one population were automatically measured from the confocal data sets. The perimeter of the cell slices was measured in the TEM images using a semi-automated segmentation… Expand
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