Semi-automated selection of cryo-EM particles in RELION-1.3

  title={Semi-automated selection of cryo-EM particles in RELION-1.3},
  author={Sjors H. W. Scheres},
  booktitle={Journal of structural biology},
The selection of particles suitable for high-resolution cryo-EM structure determination from noisy micrographs may represent a tedious and time-consuming step. Here, a semi-automated particle selection procedure is presented that has been implemented within the open-source software RELION. At the heart of the procedure lies a fully CTF-corrected template-based picking algorithm, which is supplemented by a fast sorting algorithm and reference-free 2D class averaging to remove false positives… CONTINUE READING
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