Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast
@article{Duport1998SelfsufficientBO, title={Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast}, author={Catherine Duport and Roberto Spagnoli and Eric Degryse and Denis Pompon}, journal={Nature Biotechnology}, year={1998}, volume={16}, pages={186-189} }
The first two steps of the steroidogenic pathway were reproduced in Saccharomyces cerevisiae. Engineering of sterol biosynthesis by disruption of the Δ22-desaturase gene and introduction of the Arabidopsis thaliana Δ7-reductase activity and coexpression of bovine side chain cleavage cytochrome P450, adrenodoxin, and adrenodoxin reductase, lead to pregnenolone biosynthesis from a simple carbon source. Following additional coexpression of human β-hydroxysteroid dehydrogenase/isomerase…
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