Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast

@article{Duport1998SelfsufficientBO,
  title={Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast},
  author={Catherine Duport and Roberto Spagnoli and Eric Degryse and Denis Pompon},
  journal={Nature Biotechnology},
  year={1998},
  volume={16},
  pages={186-189}
}
The first two steps of the steroidogenic pathway were reproduced in Saccharomyces cerevisiae. Engineering of sterol biosynthesis by disruption of the Δ22-desaturase gene and introduction of the Arabidopsis thaliana Δ7-reductase activity and coexpression of bovine side chain cleavage cytochrome P450, adrenodoxin, and adrenodoxin reductase, lead to pregnenolone biosynthesis from a simple carbon source. Following additional coexpression of human β-hydroxysteroid dehydrogenase/isomerase… 
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