Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast

  title={Self-sufficient biosynthesis of pregnenolone and progesterone in engineered yeast},
  author={C. Duport and R. Spagnoli and E. Degryse and D. Pompon},
  journal={Nature Biotechnology},
  • C. Duport, R. Spagnoli, +1 author D. Pompon
  • Published 1998
  • Biology, Medicine
  • Nature Biotechnology
  • The first two steps of the steroidogenic pathway were reproduced in Saccharomyces cerevisiae. Engineering of sterol biosynthesis by disruption of the Δ22-desaturase gene and introduction of the Arabidopsis thaliana Δ7-reductase activity and coexpression of bovine side chain cleavage cytochrome P450, adrenodoxin, and adrenodoxin reductase, lead to pregnenolone biosynthesis from a simple carbon source. Following additional coexpression of human β-hydroxysteroid dehydrogenase/isomerase… CONTINUE READING
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